CellGenix® rh HGF
Preclinical grade
CellGenix Recombinant Human HGF reliably stimulates the differentiation of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) into hepatocytes, endothelial and epithelial cells, melanocytes and keratinocytes.
General Information
Hepatocyte growth factor (HGF) is a potent mitogen that plays a role in the induction of cell proliferation, motility, morphogenesis and inhibition of cell growth.
CellGenix Recombinant Human HGF is produced using a human amniocyte cell line (CAP®). By using the CAP® technology we are able to produce this difficult to express glyco-protein with authentic human post-translational modifications. These human post-translational modifications, e.g. glycosylation, are not accessible by bacterial expression.
Preclinical Grade
The final manufacturing steps and quality control of all our preclinical grade products are performed in a GMP facility ensuring a consistent high quality.
name
CellGenix® Recombinant Human Hepatocyte Growth Factor
Source
CAP® cell line
Formulation
Lyophilized
ANIMAL-DERIVED COMPONENT-FREE
CellGenix® Preclinical rh HGF
CellGenix® rh HGF
Features
- Contains neither animal- nor human-derived materials
- High purity, activity and product stability
- Authentic human glycosylation pattern
Applications
- Differentiation of ESCs and iPSCs into hepatocytes, endothelial and epithelial cells, melanocytes and keratinocytes
Technical Details
Source
Expressed in CAP® cell line
Description
Hepatocyte Growth Factor (HGF)
Formulation
Lyophilized from a 0.2 µm-filtered solution containing 10 mM Na-phosphate and 400 mM NaCl
Final manufacturing steps and Quality Control (QC) are performed in a GMP facility.
| | Preclinical |
| Molecular weight | 79.6 kDa |
| Purity | ≥ 95% as determined by SDS-PAGE |
| Activity | ≥ 0.5 x 106 IU/mg, calibrated against NIBSC #96/564 Measured by its ability to induce IL-11 secretion of Saos-2 ce |
| Endotoxin level | < 1000 EU/mg |
| Intended use | Intended for preclinical ex vivo use. Not intended for therapeutic use |
Handling Instructions
Reconstitution
Recommended in sterile water to a final concentration of 100 µg/ml.
Shipment
Ambient temperature. Please refer to Technote to learn more about our shipment validation procedure.
Expiry
2 years from date of shipment.
Storage & Stability
Store lyophilized cytokine at -20°C to -80°C.
Avoid repeated freeze/thaw cycles.
