Technical Details
Human EGF, accession # P01133, Asn971-Arg1023
Lyophilized from a 0.2 µm-filtered solution containing 25 mM sodium acetate, pH 5.0
Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP.
| Preclinical | GMP | |
| Molecular weight | 7.2 kDa | 7.2 kDa |
| Purity | ≥ 95% as determined by RP-HPLC | ≥ 97% as determined by RP-HPLC |
| Activity | ≥ 0.5 x 106 IU/mg, calibrated against NIBSC #91/530 Measured in a cell proliferation assay using an EGF-dependent cell line, Balb/3T3 |
0.5 – 2.0 x 106 IU/mg, calibrated against NIBSC #91/530 Measured in a cell proliferation assay using an EGF-dependent cell line, Balb/3T3 Batch specific activity on CoA |
| Endotoxin level | < 1000 EU/mg | ≤ 50 EU/mg |
| Intended use | Intended for preclinical ex vivo use. Not intended for therapeutic use. | Intended for clinical ex vivo use. Not intended for human in vivo application. |
Handling Instructions
Recommended in 0.2% acetic acid to a final concentration of 250 µg/ml for 50 µg vials or 500 µg/ml for 1 mg vials.
Ambient temperature. Please refer to Technote to learn more about our shipment validation procedure.
≥ 6 months from date of shipping. Please refer to Certificate of Analysis for the exact expiry date.
Store lyophilized cytokine at -20°C to -80°C.
Store a 250 µg/ml cytokine solution
• 4 weeks at 2°C to 8°C under sterile conditions after reconstitution. Store in the original container.
• 4 months at -20°C to -80°C under sterile conditions after reconstitution. Store in 60 µl aliquots in polypropylene cryogenic vials.
Avoid repeated freeze/thaw cycles.
