Technical Details
Source
Expressed in E. coli
Description
Human Interferon-gamma, accession # P01579, Gln24-Arg162
Formulation
Lyophilized from a 0.2 µm-filtered solution containing 20 mM succinate, pH 5.0
Final manufacturing steps and Quality Control (QC) are performed in a GMP facility.
| Preclinical | |
| Molecular weight | 16.5 kDa |
| Purity | ≥ 95% as determined by SDS-PAGE |
| Activity | ≥ 10 x 106 IU/mg, calibrated against Imukin® Measured in a cell cytotoxicity assay using HT-29 cells |
| Endotoxin level | < 1000 EU/mg |
| Intended use | Intended for preclinical ex vivo use. Not intended for therapeutic use. |
Handling Instructions
Reconstitution
Recommended in sterile water to a final concentration of 250 µg/ml.
Shipment
Ambient temperature. Please refer to our Technote Shipment of CellGenix® Preclinical and GMP Cytokines at Ambient Temperatures.
Expiry
3 years from date of shipment.
Storage & Stability
Store lyophilized cytokine at -20°C to -80°C.
Avoid repeated freeze/thaw cycles.