Human TNF-α, accession # P01375, Val77-Leu233
Lyophilized from 200 µl of a 0.2 µm-filtered solution containing 1.5 mM potassium phosphate, 8.1 mM sodium phosphate, 2.7 mM potassium chloride and 137 mM sodium chloride, pH 7.5
Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP.
|Molecular weight||17.5 kDa||17.5 kDa|
|Purity||≥ 95% as determined by SDS-PAGE||≥ 97% as determined by SDS-PAGE|
|Activity||≥ 20 x 106 IU/mg, calibrated against NIBSC #88/786
Measured in a cell cytotoxicity assay using a TNF-α-sensitive cell line, L929
20 – 80 x 106 IU/mg, calibrated against NIBSC #88/786
Batch specific activity on CoA
|Endotoxin level||< 1000 EU/mg||≤ 50 EU/mg|
|Intended use||Intended for preclinical ex vivo use. Not intended for therapeutic use.||Intended for clinical ex vivo use. Not intended for human in vivo application.|
Recommended in sterile water to a final concentration of 250 µg/ml.
Ambient temperature. Please refer to our Technote Shipment of CellGenix® Preclinical and GMP Cytokines at Ambient Temperatures.
≥ 6 months from date of shipping. Please refer to Certificate of Analysis for the exact expiry date.
Store lyophilized cytokine at -20°C to -80°C.
Store a 100 µg/ml reconstituted cytokine solution:
• 4 weeks at 2°C to 8°C under sterile conditions after reconstitution. Store in the original container.
Store a 250 µg/ml reconstituted cytokine solution:
• 4 months at -20°C to -80°C under sterile conditions after reconstitution. Store in 80 µl aliquots in polypropylene cryogenic vials.
Avoid repeated freeze/thaw cycles.