Technical Details
Human Interleukin-7, accession # P13232, Asp26-His177
Lyophilized from 200 µl of a 0.2 µm-filtered solution containing 25 mM sodium phosphate, 150 mM sodium chloride and 0.1 % polysorbate 20, pH 7.4
Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP.
| Preclinical | GMP | |
| Molecular weight | 18.3 kDa | 18.3 kDa |
| Purity | ≥ 95% as determined by SDS-PAGE | ≥ 97% as determined by SDS-PAGE |
| Activity | ≥ 50 x 106 IU/mg, calibrated against NIBSC #90/530 Measured in a cell proliferation assay using an IL-7-dependent cell line, IxN/2b | 50 – 200 x 106 IU/mg, calibrated against NIBSC #90/530 Measured in a cell proliferation assay using an IL-7-dependent cell line, IxN/2b
Batch specific activity on CoA
|
| Endotoxin level | ≤ 25 EU/mg | ≤ 25 EU/mg |
| Intended use | Intended for preclinical ex vivo use. Not intended for therapeutic use. | Intended for clinical ex vivo use. Not intended for human in vivo application. |
Handling Instructions
Recommended in sterile water to a final concentration of 250 µg/ml for 50 µg vials.
Ambient temperature. Please refer to our Technote Shipment of CellGenix® preclinical and GMP cytokines at ambient temperatures.
GMP: ≥ 6 months from date of shipping
Preclinical: 3 years from date of shipping
Please refer to Certificate of Analysis for the exact expiry date.
Store lyophilized cytokine at -20°C to -80°C.
Store a 250 µg/ml reconstituted cytokine solution:
• 4 weeks at 2°C to 8°C under sterile conditions after reconstitution. Store in the original container.
• 4 months at -20°C to -80°C under sterile conditions after reconstitution. Store in aliquots in polypropylene cryogenic vials.
Avoid repeated freeze/thaw cycles.
