Technical Details
Human IL-3, accession # P08700, Ala20-Phe152
Lyophilized from a 200 µl of a 0.2 µm-filtered solution containing 10 mM sodium phosphate and 50 mM sodium chloride, pH 7.2
Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP.
| Preclinical | GMP | |
| Molecular weight | 15.2 kDa | 15.2 kDa |
| Purity | ≥ 95% as determined by SDS-PAGE | ≥ 97% as determined by SDS-PAGE and RP-HPLC |
| Activity |
≥ 0.9 x 106 IU/mg, calibrated against NIBSC #91/510 |
0.9 – 3.6 x 106 IU/mg, calibrated against NIBSC #91/510 Measured in a cell proliferation assay using an IL-3-dependent cell line, TF-1 Batch specific activity on CoA |
| Endotoxin level | < 1000 EU/mg | ≤ 50 EU/mg |
| Intended use | Intended for preclinical ex vivo use. Not intended for therapeutic use. | Intended for clinical ex vivo use. Not intended for human in vivo application. |
Handling Instructions
Recommended in sterile water to a final concentration of 250 µg/ml.
Ambient temperature. Please refer to our Technote Shipment of CellGenix® Preclinical and GMP Cytokines at Ambient Temperatures.
≥ 6 months from date of shipping. Please refer to Certificate of Analysis for the exact expiry date.
Store lyophilized cytokine at -20°C to -80°C.
Store a 100 µg/ml reconstituted cytokine solution:
• 4 weeks at 2°C to 8°C under sterile conditions after reconstitution. Store in the original container.
Store a 250 µg/ml reconstituted cytokine solution:
• 4 months at -20°C to -80°C under sterile conditions after reconstitution. Store in aliquots in polypropylene cryogenic vials.
Avoid repeated freeze/thaw cycles.
