VueLife® AC Bags

Adherent Cell Culture Bags

VueLife AC Bags are designed to culture adherent cells in a closed system environment. They can also be used for the expansion of cord blood.

General Information

All VueLife AC bags are made from the highest quality USP Class VI fluorinated ethylene propylene (FEP) material. They provide high permeability to oxygen and carbon dioxide whilst remaining impermeable to water for improved culture and expansion. The unique design allows for culture in a closed system environment with the use of sterile docking equipment. Each VueLife adherent cell culture bag is provided with standard PVC tubing.

VueLife AC Bags utilize a treated form of FEP film giving the interior of the bag a higher surface energy than that of the VueLife C Bags. This modified surface energy is meant to better promote cell and protein adhesion to the surface of the bag.

To offer a maximum of flexibility the VueLife AC Bags are available in different cell culture volumes ranging from 32 ml to 750ml.

Product Documents
Order Information
Inquiries

Name

VueLife® Adherent Cell Culture Bags, AC series

FEP Material

Fluorinated ethylene propylene (FEP) film; 5 mil (.005”)
USP Class VI compliant
Optically clear, transmits UV, visible and IR light
Non-immunogenic; FEP material has no extractables, compliant with USP <661>

Tubing

Pre-attached PVC tubing leading to a female luer port (typically Y-tubing with additional sterile docking tube), USP Class VI material

Animal-derived component free

Film and ports are animal-derived component-free (ADCF)

Products

VueLife® AC Bags:
32-AC, 72-AC, 118-AC, 119-AC, 197-AC, 290-AC, 750-AC

VueLife® AC Bags

Features

Unique FEP film with proprietary surface treatment permits reversible adherence of cells and proteins
Connection via sterile docking to the PVC tubing or a female luer lock
Safe and aseptic access through Female Luer Valve (needle-less injection site which mates with standard male luer fittings or needle adapters)
Culture volumes can be gradually expanded during cell culture by using external clamps
Non-reactive with virtually all chemicals and biologics, including DMSO and DMF

Applications

Culture of cells that may require adhesion, e.g. maturation of dendritic cells
Culture of cells under circumstances that require a protein layer, e.g. for transfection
Sterile expansion of cord blood

Adherence specific feature

Inner surface layer is treated by corona discharge which leads to enhanced surface energy and as a result to adherence. This can be reversed by using conventional methods (e.g. trypsin) or by physical measures (infusing sterile air into the bag). After sterile air infusion the interior surface needs to dry. Drying takes less than one minute and is readily apparent by visual observation. To release all cells the bag has to be turned over to allow the air to dry on both sides.

VueLife® Adherent Cell Culture (AC) Bags

Name	VueLife® 32-AC Bag	VueLife® 72-AC Bag	VueLife® 118-AC Bag	VueLife® 119-AC Bag
Cell Culture Volume (at 1 cm thick)	32 ml	72 ml	118 ml	118 ml
Order Number	3330 (Pack of 5)	3331 (Pack of 5)	3332 (Pack of 5)	3336 (Pack of 5)
Outside Dimensions	9.1 cm x 17 cm	9.7 cm x 22.4 cm	21.8 cm x 13.7 cm	21.8 cm x 14.7 cm
Inside Dimensions	8.6 cm x 5.8 cm	8.6 cm x 11.2 cm	10.4 cm x 12.8 cm	10.4 cm x 13.7 cm
Inside Surface Area (2 sides)	100 cm²	193 cm²	266 cm²	285 cm²
Permeability (cc/day@37°C) 0₂ (gas)	31	59	82	88
CO₂ (gas)	71	137	198	202
N₂ (gas)	12	22	31	33
H₂O (gas)	0.006	0.01	0.02	0.02
Ports on Bag	1 Swabable Female Luer Valve (Needleless Injection Site)	1 Swabable Female Luer Valve (Needleless Injection Site)	1 Swabable Female Luer Valve (Needleless Injection Site)	1 Swabable Female Luer Valve (Needleless Injection Site)
Ports on Tubing	1 Female Luer Lock 1 Blind End	1 Female Luer Lock 1 Blind End	1 Female Luer Lock 1 Blind End	1 Female Luer Lock 1 Blind End

VueLife® Adherent Cell Culture (AC) Bags

Name	VueLife® 197-AC Bag	VueLife® 290-AC Bag	VueLife® 750-AC Bag
Cell Culture Volume (at 1 cm thick)	197 ml	290 ml	750 ml
Order Number	3333 (Pack of 5)	3334 (Pack of 5)	3335 (Pack of 5)
Outside Dimensions	14.9 cm x 24.4 cm	14.2 cm x 37.1 cm	22.1 cm x 53 cm
Inside Dimensions	23.4 cm x 14.2 cm	13.2 cm x 26.7 cm	20.8 cm x 38.6 cm
Inside Surface Area (2 sides)	665 cm²	705 cm²	1606 cm²
Permeability (cc/day@37°C) 0₂ (gas)	150	217	495
CO₂ (gas)	347	501	1140
N₂ (gas)	56	81	186
H₂O (gas)	0.03	0.04	0.10
Ports on Bag	1 Swabable Female Luer Valve (Needleless Injection Site)	1 Swabable Female Luer Valve (Needleless Injection Site)	1 Female Luer Lock 1 Swabable Female Luer Valve (Needleless Injection Site)
Ports on Tubing	1 Female Luer Lock 1 Blind End	1 Female Luer Lock 1 Blind End	1 Female Luer Lock 1 Blind End

Documents

Handling Instructions
Certificate of Conformance (on request)

Regulatory Support

We offer the following to assist you with your regulatory approval process:

Made from the highest quality USP Class VI materials
Separate Certificate of Sterilization for each lot
Change notifications prior to relevant changes

Please contact our Regulatory Support Team for all your regulatory requests & questions:
Phone: +49 761 88 88 9 – 0
Email: regulatorysupport@cellgenix.com