Name
 
 
VueLife® Adherent Cell Culture Bags, AC series
FEP Material
 
 
  • Fluorinated ethylene propylene (FEP) film; 5 mil (.005”)
  • USP Class VI compliant
  • Optically clear, transmits UV, visible and IR light
  • Non-immunogenic; FEP material has no extractables, compliant with USP <661>
Tubing
 
 
Pre-attached PVC tubing leading to a female luer port (typically Y-tubing with additional sterile docking tube), USP Class VI material
Animal-derived component free
 
 
Film and ports are animal-derived component-free (ADCF)
Products
 
 

VueLife® AC Bags:
32-AC, 72-AC, 118-AC, 119-AC, 197-AC, 290-AC, 750-AC

VueLife® AC Bags

Features

  • Unique FEP film with proprietary surface treatment permits reversible adherence of cells and proteins
  • Connection via sterile docking to the PVC tubing or a female luer lock
  • Safe and aseptic access through Female Luer Valve (needle-less injection site which mates with standard male luer fittings or needle adapters)
  • Culture volumes can be gradually expanded during cell culture by using external clamps
  • Non-reactive with virtually all chemicals and biologics, including DMSO and DMF

Applications

  • Culture of cells that may require adhesion, e.g. maturation of dendritic cells
  • Culture of cells under circumstances that require a protein layer, e.g. for transfection
  • Sterile expansion of cord blood

Adherence specific feature

Inner surface layer is treated by corona discharge which leads to enhanced surface energy and as a result to adherence. This can be reversed by using conventional methods (e.g. trypsin) or by physical measures (infusing sterile air into the bag). After sterile air infusion the interior surface needs to dry. Drying takes less than one minute and is readily apparent by visual observation. To release all cells the bag has to be turned over to allow the air to dry on both sides.

VueLife® Adherent Cell Culture (AC) Bags

Name

VueLife® 32-AC Bag

VueLife® 72-AC Bag

VueLife® 118-AC Bag

VueLife® 119-AC Bag

Cell Culture Volume (at 1 cm thick)

32 ml 72 ml 118 ml 118 ml

Order Number

3330 (Pack of 5) 3331 (Pack of 5) 3332 (Pack of 5) 3336 (Pack of 5)

Outside Dimensions

9.1 cm x 17 cm

9.7 cm x 22.4 cm

21.8 cm x 13.7 cm

21.8 cm x 14.7 cm

Inside Dimensions

8.6 cm x 5.8 cm

8.6 cm x 11.2 cm

10.4 cm x 12.8 cm

10.4 cm x 13.7 cm

Inside Surface Area (2 sides)

100 cm²

193 cm²

266 cm²

285 cm²

Permeability  (cc/day@37°C)

02 (gas)

 

31

 

59

 

82

 

88

CO2 (gas)

71

137

198

202

N2 (gas)

12

22

31

33

H2O (gas)

0.006

0.01

0.02

0.02

Ports on Bag

1 Swabable Female Luer Valve (Needleless Injection Site)

1 Swabable Female Luer Valve (Needleless Injection Site)

1 Swabable Female Luer Valve (Needleless Injection Site)

1 Swabable Female Luer Valve (Needleless Injection Site)

 Ports on Tubing

1 Female Luer Lock

1 Blind End

 1 Female Luer Lock

1 Blind End

 1 Female Luer Lock

1 Blind End

1 Female Luer Lock

1 Blind End

 

VueLife® Adherent Cell Culture (AC) Bags

Name

VueLife® 197-AC Bag VueLife® 290-AC Bag VueLife® 750-AC Bag

Cell Culture Volume (at 1 cm thick)

197 ml 290 ml 750 ml

Order Number

3333 (Pack of 5)

3334 (Pack of 5)

3335 (Pack of 5)

Outside Dimensions

14.9 cm x 24.4 cm

14.2 cm x 37.1 cm

22.1 cm x 53 cm

Inside Dimensions

23.4 cm x 14.2 cm

13.2 cm x 26.7 cm

20.8 cm x 38.6 cm

Inside Surface Area (2 sides)

665 cm²

705 cm²

1606 cm²

Permeability  (cc/day@37°C)

02 (gas)

 

150

 

217

 

495

CO2 (gas)

347

501

1140

N2 (gas)

56

81

186

H2O (gas)

0.03

0.04

0.10

Ports on Bag

1 Swabable Female Luer Valve (Needleless Injection Site)

1 Swabable Female Luer Valve (Needleless Injection Site)

1 Female Luer Lock

1 Swabable Female Luer Valve (Needleless Injection Site)

 Ports on Tubing

1 Female Luer Lock

1 Blind End

 1 Female Luer Lock

1 Blind End

1 Female Luer Lock

1 Blind End

Documents

Regulatory Support

We offer the following to assist you with your regulatory approval process:

  • Made from the highest quality USP Class VI materials
  • Separate Certificate of Sterilization for each lot
  • Change notifications prior to relevant changes

Please contact our Regulatory Support Team for all your regulatory requests & questions:
Phone:     +49 761 88 88 9 – 0
Email:      regulatorysupport@cellgenix.com

Publications

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