In this application note, we show how enhanced T cell expansion along with the generation of an early differentiated phenotype can be achieved using the G-Rex® system with CellGenix® serum-free reagents, such as CellGenix® T cell Medium (CellGenix® TCM) supplemented with CellGenix® IL-7 and IL-15.
Using serum-free reagents in your manufacturing process removes potential risk and variability introduced when using human serum, yielding a product with a low proportion of PD-1+ cells. The G-Rex® system reduces handling steps for more efficient production, eliminating the need to change media and refeed cytokines over an 11-day culture. The system is scalable, closed system versions are available, and it is widely used in clinical settings.
You can download the application note here.