CellGenix® rh TGF-β1

Preclinical grade

CellGenix Recombinant Human TGF-β1 reliably promotes the expansion of induced pluripotent stem cells, embryonic stem cellsas well as mesenchymal stem cells. It also stimulates the differentiation of T cells into regulatory T cells and the expansion of regulatory T cells. Proper glycosylation of TGF-ß1 is of significant importance for the protein’s processing and functional expression – one of the reasons why production of this growth factor and certain complexly glycosylated proteins is not feasible using conventional bacterial expression systems.

General Information

Transforming Growth Factor-beta 1 (TGF-β1) is a member of the growth factor beta superfamily and plays a role in various cellular functions like cell differentiation, proliferation and apoptosis. It is secreted by most immune cells and especially interacts with T cells, B cells, macrophages and monocytes.

CellGenix Recombinant Human TGF-β1 is produced using a human amniocyte cell line (CAP®). By using the CAP® technology we are able to produce this difficult to express glyco-protein with authentic human post-translational modifications. These human post-translational modifications are not accessible by bacterial expression.

name
CellGenix® Recombinant Human Transforming Growth Factor-beta 1
Source
CAP® cell line
Formulation
Lyophilized
ANIMAL-DERIVED COMPONENT-FREE
CellGenix® Preclinical rh TGF-β1
amount
Order Number
50  µg
1426-050

CellGenix® rh TGF-β1

Features

  • Preclinical grade – will be further developed to GMP
  • The final product contains neither animal- nor human-derived materials
  • High activity, purity and product stability

Applications

  • Differentiation of T cells into regulatory T cells (Treg)
  • Expansion of Treg cells
  • Expansion of embryonic stem cells (ESCs)
  • Expansion of induced pluripotent stem cells (iPSCs)
  • Expansion of mesenchymal stem cells (MSCs)

Technical Details

Source
Expressed in CAP® cell line
Description

Human Transforming Growth Factor-beta 1, accession # PO1137, Ala279-Ser390

Formulation

Lyophilized from a 0.2 µm-filtered solution containing 1% Mannitol

The production cell line was derived from an extensively characterized human amniocyte cell line (CAP®).

 Preclinical
Molecular weight25.6 kDa
Purity≥ 90%1 as determined by SDS-PAGE
Activity≥ 9 x 106 IU/mg, calibrated against NIBSC #89/514
Endotoxin level< 10 EU/mg
Intended useIntended for preclinical ex vivo use. Not intended for therapeutic use

1 May contain up to 2% of Insulin-like growth factor-binding protein 2 (IGFBP-2) as a host cell-derived impurity.

Handling Instructions

Reconstitution

Recommended in sterile water to a final concentration of 250 µg/ml.

Shipment

Ambient temperature. Please refer to Technote to learn more about our shipment validation procedure.

Expiry

3 years from date of shipment.

Storage & Stability

Store lyophilized cytokine at -20°C to -80°C.

Avoid repeated freeze/thaw cycles.

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