CellGenix® rh IL-6

GMP and Preclinical grade

CellGenix Recombinant Human IL-6 reliably stimulates the maturation of immature to mature dendritic cells (DCs) and promotes the proliferation of cord blood derived hematopoietic stem cells (HSCs/CD133+ cells). It is produced in our dedicated animal-free facility ensuring maximum safety for optimal use in ATMP manufacturing.

General Information

Interleukin-6 (IL-6) is a multifunctional cytokine that acts both pro-inflammatory and anti-inflammatory. It regulates acute phase responses, inflammatory reactions, hematopoiesis, bone metabolism, cancer progression and immune responses. It is produced primarily by T cells, monocytes and macrophages.

IL-6 is used in the cell and gene therapy space for the ex vivo maturation of immature DCs and proliferation of HSCs.

GMP Grade

CellGenix Recombinant Human IL-6 is produced following all applicable GMP guidelines and allows for the safe use in accordance with USP Chapter <1043> and Ph. Eur. General Chapter 5.2.12.

Our GMP cytokines are provided with documented evidence of purity, potency, consistency and stability. In combination with our expert regulatory and technical support this will help simplify your raw material qualification and validation efforts.

name
CellGenix® Recombinant Human Interleukin-6
Source
E. coli
Formulation
Lyophilized
ANIMAL-DERIVED COMPONENT-FREE
CellGenix® GMP rh IL-6
amount
Order Number
50  µg
1004-050
1 mg
1004-1000
CellGenix® Preclinical rh IL-6
amount
Order Number
50  µg
1404-050

CellGenix® rh IL-6

Features

  • Produced in our dedicated animal-free facility
  • Compliant to USP Chapter <1043> and Ph. Eur. General Chapter 5.2.12 (GMP)
  • FDA Drug Master File available (GMP)

GMP & Preclinical Grade

  • Seamless transition from preclinical development to ATMP manufacturing

Applications

Highest GMP Quality Standard

  • High lot-to-lot consistency – save time on revalidations
  • Performance reliability – rely on consistent product performance
  • Highest purity – ensure the safety of your ATMP
  • Extremely low endotoxin levels – improve safety and reproducibility
  • Expert regulatory & technical support – rely on experience

Read more about our GMP Quality

 

Safe │ GMP Compliant │ Reliable

Technical Details

Source
Expressed in E. coli
Description

Human IL-6, accession # P05231, Pro29-Met212

Formulation

Lyophilized from a 0.2 µm-filtered solution containing 1.5 mM potassium phosphate, 8.1 mM sodium phosphate, 2.7 mM potassium chloride and 137 mM sodium chloride, pH 7.5

Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP

 PreclinicalGMP
Molecular weight21.9 kDa21.9 kDa
Purity≥ 95% as determined by SDS-PAGE≥ 97% as determined by SDS-PAGE
Activity≥ 50 x 106 IU/mg, calibrated against NIBSC #89/54880 – 250 x 106 IU/mg, calibrated against NIBSC #89/548
Batch specific activity on CoA
Endotoxin level< 1000 EU/mg≤ 50 EU/mg
Intended useIntended for preclinical ex vivo use. Not intended for human in vivo application.Intended for clinical ex vivo use. Not intended for human in vivo application.

Handling Instructions

Reconstitution

Recommended in sterile water to a final concentration of 250 µg/ml for 50 µg vials or 500 µg/ml for 1 mg vials. We recommend to not vortex the solution. The solution can be turbid, this however does not influence the activity. 

Shipment

Ambient temperature. Please refer to Technote to learn more about our shipment validation procedure.

Expiry

≥ 6 months from date of shipping. Please refer to Certificate of Analysis (CoA) for the exact expiry date.

Storage & Stability

Store lyophilized cytokine at -20°C to -80°C.

Avoid repeated freeze/thaw cycles.

Documents

Data

CellGenix GMP rh IL-6 has an activity of 80 – 250 × 106 IU/mg

The activity of GMP rh IL-6 was measured in a cell proliferation assay using the IL-6-dependent cell line B9. It was calibrated against the NIBSC #89/548.

You can find the batch specific activity on the certificate of analysis (CoA).

IL6, Interleukin6

Publications