CellGenix® rh HGF

Preclinical grade

CellGenix Recombinant Human HGF reliably stimulates the differentiation of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) into hepatocytes, endothelial and epithelial cells, melanocytes and keratinocytes.

General Information

Hepatocyte growth factor (HGF) is a potent mitogen that plays a role in the induction of cell proliferation, motility, morphogenesis and inhibition of cell growth.

CellGenix Recombinant Human HGF is produced using a human amniocyte cell line (CAP®). By using the CAP® technology we are able to produce this difficult to express glyco-protein with authentic human post-translational modifications. These human post-translational modifications, e.g. glycosylation, are not accessible by bacterial expression.

Preclinical Grade

The final manufacturing steps and quality control of all our preclinical grade products are performed in a GMP facility ensuring a consistent high quality.

name
CellGenix® Recombinant Human Hepatocyte Growth Factor
Source
CAP® cell line
Formulation
Lyophilized
ANIMAL-DERIVED COMPONENT-FREE
CellGenix® Preclinical rh HGF
amount
Order Number
10 µg
1424-010

CellGenix® rh HGF

Features

  • Contains neither animal- nor human-derived materials
  • High purity, activity and product stability
  • Shows a human glycosylation pattern

Applications

  • Differentiation of ESCs and iPSCs into hepatocytes, endothelial and epithelial cells, melanocytes and keratinocytes

Technical Details

Source
Expressed in CAP® cell line
Description

Human HGF, accession #

Formulation

Lyophilized from a 0.2 µm-filtered solution containing 10 mM Na-phosphate and 400 mM NaCl

Final manufacturing steps and Quality Control (QC) are performed in a GMP facility.

 Preclinical
Molecular weight79.6 kDa
Purity≥ 95% as determined by SDS-PAGE
Activity≥ 0.5 x 106 IU/mg, calibrated against NIBSC #96/564
Endotoxin level< 1000 EU/mg
Intended useIntended for preclinical ex vivo use. Not intended for human in vivo application.

Handling Instructions

Reconstitution

Recommended in sterile water to a final concentration of 100 µg/ml.

Shipment

Ambient temperature. Please refer to Technote to learn more about our shipment validation procedure.

Expiry

2 years from date of shipment.

Storage & Stability

Store lyophilized cytokine at -20°C to -80°C.

Avoid repeated freeze/thaw cycles.

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